cag promoter (737 and a t-cell epitope region (495)  (GenScript corporation)

Journal: Virology Journal

Article Title: A pseudotype baculovirus expressing the capsid protein of foot-and-mouth disease virus and a T-Cell immunogen shows enhanced immunogenicity in mice

doi: 10.1186/1743-422X-8-77

Figure Lengend Snippet: Schematic diagram of the transfer plasmid for construction of recombinant baculovirus . The structure of the transfer plasmid based on pFastBac Dual is displayed. pH, polyhedrin promoter of baculovirus; p10, p10 promoter of baculovirus; VSV/GED, truncated glycoprotein of vesicular stomatitis virus including 21 amino acid ectodomain with transmembrane and cytoplasmic tail domains; CMV, cytomegalovirus immediate-early promoter/enhancer; SV40 or tk poly (A), polyadenylation signal from Simian virus 40 or tk gene of Herpes simplex virus; P12A3C, the P12A3C gene encoding structural and non-structural proteins necessary for the formation of FMDV empty capsid; CAG, a composite promoter consisting of the CMV IE enhancer and chicken β-actin promoter; T-cell epitopes including a pan DR epitope sequence (PADRE), seven T cell epitope regions in structural or non-structural proteins of FMDV and a invasin immunostimulatory sequence taken from Yersinia, as described by Wang et al. (2002).

Article Snippet: The CAG promoter (737 bp) and a T-cell epitope region (495 bp) were synthesized by GenScript Corporation and inserted into pFastBacDual-GED-P12A3C downstream of the p10 promoter followed by the HSV tk Poly (A) tail.

Techniques: Plasmid Preparation, Recombinant, Virus, Sequencing